5KZP

Structure of the HCV1-C1 Antibody-Antigen Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 1
Unit cell lengths	47.070, 94.538, 126.758
Unit cell angles	91.84, 94.95, 97.91

Refinement procedure

Resolution	29.490 - 2.262
R-factor	0.2202
Rwork	0.218
R-free	0.26110
RMSD bond length	0.012
RMSD bond angle	1.587
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.10pre_2124)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.490
High resolution limit [Å]	2.260
Rmerge	0.069
Number of reflections	186500
<I/σ(I)>	11.4
Completeness [%]	92.7
Redundancy	3.5
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	0.1M MES pH 6.0 0.2M Calcium Acetate 20% PEG8000