5KZM

Crystal structure of Tryptophan synthase alpha-beta chain complex from Francisella tularensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	111.176, 171.986, 76.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.496 - 2.804
R-factor	0.1858
Rwork	0.183
R-free	0.23470
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.574
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (dev_2386)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.850
High resolution limit [Å]	2.800	7.560	2.800
Rmerge	0.132	0.057	0.981
Rmeas	0.143	0.064
Rpim	0.067	0.030	0.491
Total number of observations	87874
Number of reflections	18264
<I/σ(I)>	5.8
Completeness [%]	100.0	99.8	100
Redundancy	4.8	4.4	4.9
CC(1/2)		0.995	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M calcium acetate, 0.1M imidazole, 10% PEG 8000