5KZ9
Crystal structure of the Rous sarcoma virus matrix protein.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-02 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0332 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 66.200, 66.200, 218.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.000 - 2.850 |
R-factor | 0.19761 |
Rwork | 0.195 |
R-free | 0.23855 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.325 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.000 |
High resolution limit [Å] | 2.850 |
Rmerge | 0.070 |
Number of reflections | 6104 |
<I/σ(I)> | 34 |
Completeness [%] | 99.9 |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.3 | 293 | 2.60 M Ammonium formate, 0.20 M Beta-Alanine/KOH pH 10.3 |