5KYV

Structure of Photinus pyralis Luciferase green shifted light emitting variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	113
Detector technology	CCD
Collection date	2008-01-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9179
Spacegroup name	P 31 2 1
Unit cell lengths	93.078, 93.078, 293.645
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.063 - 2.500
R-factor	0.1967
Rwork	0.194
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g36
RMSD bond length	0.008
RMSD bond angle	1.148
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.108	0.368
Number of reflections	51916
<I/σ(I)>	10.3
Completeness [%]	99.3	99.9
Redundancy	6.3	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	23 % PEG 8000, 200 mM Na/K tartrate, 1.00 mM Tris