5KYI
Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 9 at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-12-08 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.129, 60.426, 38.446 |
| Unit cell angles | 90.00, 93.86, 90.00 |
Refinement procedure
| Resolution | 38.360 - 1.700 |
| R-factor | 0.1761 |
| Rwork | 0.175 |
| R-free | 0.20320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.813 |
| Data reduction software | CrysalisPro (1.171.36.32) |
| Data scaling software | CrysalisPro (1.171.36.32) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.018 | 0.108 |
| Number of reflections | 15727 | |
| <I/σ(I)> | 55.16 | 8.97 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 40% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
