5KYE
Crystal structure of USP7 catalytic domain [H294E] mutant in complex with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 43 |
Unit cell lengths | 99.550, 99.550, 83.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.892 - 1.970 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.22260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nbf |
Data reduction software | MOSFLM (7.1.1) |
Data scaling software | Aimless (0.2.8) |
Phasing software | PHASER (1.10.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 53.892 | 53.890 | 2.020 |
High resolution limit [Å] | 1.970 | 9.030 | 1.970 |
Rmerge | 0.080 | 0.023 | 0.777 |
Number of reflections | 57873 | ||
<I/σ(I)> | 12.4 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 6 | 6.9 | 5.2 |
CC(1/2) | 0.999 | 1.000 | 0.683 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.1M HEPES pH 7.5, 25% PEG3350, 0.2M Ammonium acetate |