5KY5

mouse POFUT1 in complex with EGF(+) and GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-11
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	52.080, 67.030, 111.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.156 - 1.500
R-factor	0.1583
Rwork	0.158
R-free	0.17280
RMSD bond length	0.009
RMSD bond angle	1.118
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_2341)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.109	1.092
Number of reflections	63046
<I/σ(I)>	9.5	1.7
Completeness [%]	100.0	100
Redundancy	7.3	7.3
CC(1/2)	0.994	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	20% PEG2000 MME, 50 mM Tris pH 8.5