5KY3

mouse POFUT1 in complex with mouse Factor VII EGF1 mutant (T101A) and GDP-fucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-01
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	52.150, 66.869, 110.061
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.127 - 1.530
R-factor	0.1485
Rwork	0.148
R-free	0.16360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.117
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.526	1.530
Rmerge	0.083	0.730
Number of reflections	59408
<I/σ(I)>	12.2	2.3
Completeness [%]	100.0	100
Redundancy	7.2	6.6
CC(1/2)	0.997	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	20% PEG2000 MME, 50 mM Tris pH 8.5