5KY0
mouse POFUT1 in complex with mouse Notch1 EGF12(D464G) and GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-01 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.101, 66.476, 110.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.108 - 1.530 |
| R-factor | 0.1448 |
| Rwork | 0.144 |
| R-free | 0.16410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.052 | 0.626 |
| Number of reflections | 58604 | |
| <I/σ(I)> | 20 | 2.79 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 6.8 |
| CC(1/2) | 1.000 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 25% PEG 2000 MME 50mM Tris pH8.5 |
