5KXQ

mouse POFUT1 in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-11
Detector	RAYONIX MX-225
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	56.140, 251.820, 56.080
Unit cell angles	90.00, 95.81, 90.00

Refinement procedure

Resolution	46.499 - 1.900
R-factor	0.163
Rwork	0.162
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.122
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_2341)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.036	0.421
Number of reflections	114238
<I/σ(I)>	14.2	1.9
Completeness [%]	94.0	90
Redundancy	2.2	2.1
CC(1/2)	0.999	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	1.5M malonic acid, pH 6.0