5KXN

Hen Egg White Lysozyme at 100K, Data set 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.21549
Spacegroup name	P 43 21 2
Unit cell lengths	77.311, 77.311, 37.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.655 - 1.198
R-factor	0.1601
Rwork	0.159
R-free	0.17780
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2blx
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.672	38.672	1.260
High resolution limit [Å]	1.198	3.790	1.200
Rmerge		0.030	0.240
Rmeas	0.035	0.033	0.264
Rpim	0.014	0.014	0.108
Total number of observations	238514	7499	29227
Number of reflections	35957
<I/σ(I)>	32.8	68.5	6.8
Completeness [%]	99.6	99.2	97.9
Redundancy	6.6	5.9	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	CRYSTALS WERE GROWN BY MIXING EQUAL VOLUMES OF WELL SOLUTION (1.0 M SODIUM CHLORIDE, 50 MM SODIUM ACETATE PH 4.5) AND PROTEIN (40-60 MG/ML)