5KXH

mouse POFUT1 in complex with mouse Factor VII EGF1 and GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-01
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	51.970, 66.540, 110.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.039 - 1.330
R-factor	0.1529
Rwork	0.152
R-free	0.16970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.901
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.039	1.380
High resolution limit [Å]	1.328	1.330
Rmerge	0.107	1.024
Number of reflections	87243
<I/σ(I)>	9.2	1.2
Completeness [%]	98.0	87
Redundancy	7.1	5.7
CC(1/2)	0.995	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	20% PEG 2000 MME 50mM Tris 8.5