5KWR
Crystal structure of rat Cerebellin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2015-11-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 6 |
| Unit cell lengths | 82.740, 82.740, 50.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.370 - 1.795 |
| R-factor | 0.1629 |
| Rwork | 0.160 |
| R-free | 0.18880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oum |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.740 |
| Data reduction software | XDS (Version Oct 15, 2015) |
| Data scaling software | XDS (Version Oct 15, 2015) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX ((dev_2471: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.795 | 1.800 |
| Rmerge | 0.040 | 0.644 |
| Number of reflections | 18152 | |
| <I/σ(I)> | 22.5 | 1.74 |
| Completeness [%] | 98.2 | 83.8 |
| Redundancy | 6.46 | 3.15 |
| CC(1/2) | 1.000 | 0.729 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M Tris pH 7.5, 3 M Sodium formate. Grown in the presence of Neurexin-beta |
