5KWN

The WD domain ofArabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from HY5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 2 21
Unit cell lengths	47.990, 55.176, 131.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.775 - 1.420
R-factor	0.1603
Rwork	0.160
R-free	0.17360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5igo
RMSD bond length	0.009
RMSD bond angle	1.069
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.775	1.471
High resolution limit [Å]	1.420	1.420
Rmerge		0.153
Number of reflections	66069
<I/σ(I)>	22.97	6.09
Completeness [%]	99.0	100
Redundancy	2.9	2.9
CC(1/2)		0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	Sodium Chloride 0.3M Hepes 7.2 0.1M PEG 3350 25%