5KVD

Zika specific antibody, ZV-2, bound to ZIKA envelope DIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-05-28
Detector	RDI CMOS_8M
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	39.020, 113.440, 132.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.650 - 1.650
R-factor	0.1916
Rwork	0.190
R-free	0.22120
RMSD bond length	0.004
RMSD bond angle	0.752
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.650		1.690
High resolution limit [Å]	1.650	7.380	1.650
Rmerge	0.059	0.021	0.989
Number of reflections	71401
<I/σ(I)>	18.76	61.04	2.04
Completeness [%]	98.9	94.6	100
Redundancy	6.7
CC(1/2)	0.999	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES pH6.5, 0.6 M NaCl and 20.6% PEG 4000