5KUG

Human mitochondrial calcium uniporter (residues 72-189) crystal structure with lithium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-18
Detector	RAYONIX MX-300
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	55.760, 55.760, 69.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.190 - 1.900
R-factor	0.2068
Rwork	0.204
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kue
RMSD bond length	0.008
RMSD bond angle	0.948
Data reduction software	iMOSFLM
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.130
High resolution limit [Å]	1.500
Rmerge	0.143
Number of reflections	19303
<I/σ(I)>	13.2
Completeness [%]	99.5
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.2 M lithium sulfate, 0.1 M imidazole pH 8.0, 10 % PEG 3000, 1.5 M ammonium sulfate.