5KTJ
Crystal structure of Pistol, a class of self-cleaving ribozyme
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77.15 |
Detector technology | PIXEL |
Collection date | 2016-06-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.8445, 1.0163,1.8450 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 85.190, 85.190, 256.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.270 - 2.970 |
R-factor | 0.19834 |
Rwork | 0.196 |
R-free | 0.25719 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 2.212 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.270 | |
High resolution limit [Å] | 2.971 | 2.971 |
Number of reflections | 10205 | |
<I/σ(I)> | 11.87 | |
Completeness [%] | 99.8 | |
Redundancy | 17.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 6% MPD, 0.02 M magnesium chloride, 0.02 M cobalt hexammine, 0.04 M sodium cacodylate |