5KT7

Teranry complex of human DNA polymerase iota(1-445) inserting dCMPNPP opposite template G in the presence of Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 65 2 2
Unit cell lengths	97.740, 97.740, 202.013
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.870 - 3.151
R-factor	0.2042
Rwork	0.202
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.676
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.870	50.000	3.200
High resolution limit [Å]	3.150	8.540	3.150
Rmerge	0.180	0.038	0.811
Number of reflections	10495
<I/σ(I)>	4.9
Completeness [%]	99.8	99.8	99.6
Redundancy	20.8	16.9	21.1
CC(1/2)		1.000	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	18% PEG MME 5000, 0.1M MES buffer, 0.3M ammonium sulfate