5KT2

Teranry complex of human DNA polymerase iota(26-445) inserting dCMPNPP opposite template G in the presence of Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 65 2 2
Unit cell lengths	97.605, 97.605, 203.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.017 - 2.488
R-factor	0.2215
Rwork	0.220
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.886
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.017	50.000	2.530
High resolution limit [Å]	2.488	6.750	2.490
Rmerge	0.121	0.039	0.914
Number of reflections	20810
<I/σ(I)>	6.5
Completeness [%]	99.6	99.6	99.4
Redundancy	21.3	18	22
CC(1/2)		1.000	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	13% PEG MME 5000, 0.1M MES, 0.3M ammonium sulfate