5KSV
Crystal structure of HLA-DQ2.5-CLIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 137.014, 137.014, 137.014 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.619 - 2.195 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s9v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1137) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.620 | 2.250 |
| High resolution limit [Å] | 2.195 | 2.195 |
| Rmerge | 0.129 | 1.037 |
| Number of reflections | 21943 | |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 99.7 | 97 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M BIS-TRIS, pH 5.5, 22% PEG 3350 |
