5KS9
Bel502-DQ8-glia-alpha1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.560, 56.870, 232.050 |
| Unit cell angles | 90.00, 92.77, 90.00 |
Refinement procedure
| Resolution | 46.900 - 2.550 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.160 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.900 |
| High resolution limit [Å] | 2.550 |
| Number of reflections | 63405 |
| <I/σ(I)> | 5.52 |
| Completeness [%] | 98.8 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M Na-Acetate, 22% PEG8000, Tris/Cl pH8 |
