5KRP
Frutapin, a lectin from Artocarpus incisa: Cloning, Expressing and Structural Analysis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.500, 93.680, 97.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.480 - 1.580 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.19952 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4akd |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.948 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.770 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.118 | 0.118 |
| Number of reflections | 85305 | |
| <I/σ(I)> | 8.6 | 8.6 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.996 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Potassium phosphate monobasic, PEG 8000, Glycerol |
