5KP7
Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with Holo Donor-ACP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.117, 101.117, 104.595 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.570 - 1.600 |
| R-factor | 0.1518 |
| Rwork | 0.151 |
| R-free | 0.16680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5KP5 and 5KP6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.537 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 87.570 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.116 |
| Number of reflections | 81779 |
| <I/σ(I)> | 16.9 |
| Completeness [%] | 100.0 |
| Redundancy | 19.8 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 10% PEG 8000, 120 mM (NH4)2SO4, 1X MMT pH 6.5 |
