5KO2
Mouse pgp 34 linker deleted mutant Hg derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.185, 114.741, 375.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.536 - 3.300 |
| R-factor | 0.2444 |
| Rwork | 0.243 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m1m |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (dev_2443) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.109 | 50.000 | 3.380 |
| High resolution limit [Å] | 3.300 | 8.130 | 3.300 |
| Rmerge | 0.148 | 0.104 | 0.968 |
| Rmeas | 0.154 | ||
| Rpim | 0.042 | ||
| Total number of observations | 1309474 | ||
| Number of reflections | 114118 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 92.2 | 97.6 | 89.4 |
| Redundancy | 11.5 | 14.1 | 8.3 |
| CC(1/2) | 0.994 | 0.217 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | Protein is pre-incubated with p-chloromercury phenylsulfonate to a final concentration of 4 mM of the organo mercury compound. To 10.5 ul of protein/organomercury, 3.5 ul of the precipitant 25 mM HEPES 7.5 pH, 100 mM NaCl, 0.3 mM Cymal -7, 28% PEG400 was added. Reservoir: 50 mM HEPES 7.5pH, 100 mM NaCl and 30% PEG400. |
