5KNI
Crystal Structure of the wild-type SAM domain of human Tankyrase-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.322, 55.101, 92.441 |
| Unit cell angles | 90.00, 115.88, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.17042 |
| Rwork | 0.168 |
| R-free | 0.21218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h8m |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 30719 |
| <I/σ(I)> | 10.7 |
| Completeness [%] | 98.4 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M magnesium formate |
