5KN5
TGFalpha/Epiregulin complex with neutralizing antibody LY3016859
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.249, 164.346, 64.972 |
| Unit cell angles | 90.00, 104.21, 90.00 |
Refinement procedure
| Resolution | 24.995 - 2.800 |
| Rwork | 0.206 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 24.995 | 24.995 | 2.950 |
| High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
| Rmerge | 0.028 | 0.612 | |
| Rmeas | 0.149 | ||
| Rpim | 0.077 | ||
| Total number of observations | 117046 | ||
| Number of reflections | 31162 | ||
| <I/σ(I)> | 9.6 | 21 | 1.3 |
| Completeness [%] | 98.5 | 91.8 | 99.9 |
| Redundancy | 3.8 | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 100mM MES pH 6, 14% PEG 3350, 200mM Sodium Sulfate |
