5KLB

Crystal structure of the CavAb voltage-gated calcium channel(wild-type, 2.7A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	124.900, 125.700, 191.485
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.932 - 2.700
R-factor	0.2212
Rwork	0.219
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ms2
RMSD bond length	0.013
RMSD bond angle	1.613
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.114	0.984
Number of reflections	770488
<I/σ(I)>	13.4	2.4
Completeness [%]	92.5	97.8
Redundancy	10.1	9.8
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate