5KKV

Metal-mediated coiled-coil GCN4-p2L peptide assembly crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-01
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.033
Spacegroup name	P 3 2 1
Unit cell lengths	38.328, 38.328, 46.165
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.950 - 1.900
R-factor	0.227
Rwork	0.226
R-free	0.24050
Structure solution method	SAD
RMSD bond length	0.001
RMSD bond angle	0.390
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	3324
<I/σ(I)>	23.7
Completeness [%]	99.7
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	7.1	295	20mM MOPS buffer, pH 7.1