5KIM

PSEUDO T4 LYSOZYME MUTANT - Y88PHE-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-20
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	60.283, 60.283, 96.197
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.374 - 1.500
R-factor	0.1619
Rwork	0.160
R-free	0.18440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.005
RMSD bond angle	0.817
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.058	0.043	0.341
Rmeas	0.060
Rpim	0.014
Total number of observations	431722
Number of reflections	33148
<I/σ(I)>	23.4
Completeness [%]	99.9	99.4	99.1
Redundancy	13	21.6	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	291.15	2.0M KPO4, 50mM 2-HYDROXYETHYLDISULFID, 50mM 2-MERCAPTOETHANOL, PH6.9