5KIL
CmlA beta-hydroxylase E377D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 153.954, 153.954, 92.548 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.490 - 2.720 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.26200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4jo0 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.969 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.7.0029) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.770 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.102 | 0.471 |
| Number of reflections | 30307 | |
| <I/σ(I)> | 29 | 4.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.2 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20000, potassium acetate, HEPES, glycerol |
