5KII

PSEUDO T4 LYSOZYME MUTANT - Y88PHE-METHYL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-01
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	60.183, 60.183, 95.834
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.787 - 1.560
R-factor	0.2014
Rwork	0.200
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.006
RMSD bond angle	0.774
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.590
High resolution limit [Å]	1.560	4.230	1.560
Rmerge	0.065	0.037	0.783
Rmeas	0.068
Rpim	0.017
Total number of observations	361463
Number of reflections	29084
<I/σ(I)>	18
Completeness [%]	99.1	100	98
Redundancy	12.4	26.9	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	291.15	2.0M KP04, 50mM 2-HYDROXYETHYLDISULFIDE, 50mM 2-MERCAPTOETHANOL, PH 6.9