5KI2

PSEUDO T4 LYSOZYME MUTANT - Y18PHE-METHYL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-06
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	60.197, 60.197, 95.969
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.760 - 1.500
R-factor	0.19
Rwork	0.188
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.006
RMSD bond angle	1.014
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (DEV_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.760	1.550
High resolution limit [Å]	1.499	1.499
Rmerge	0.115	0.757
Number of reflections	31673
<I/σ(I)>	23.1	1.8
Completeness [%]	96.0	67
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	291.15	2.2M KPO4, 50MM 2-HYDROXYETHYLDISULFIDE, 50MM 2-MERCAPTOETHANOL, PH 6.8