5KHT
Crystal structure of the N-terminal fragment of tropomyosin isoform Tpm1.1 at 1.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.970, 36.470, 36.710 |
| Unit cell angles | 65.21, 74.71, 78.98 |
Refinement procedure
| Resolution | 34.549 - 1.496 |
| R-factor | 0.2094 |
| Rwork | 0.205 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Chimeric model built by Phyre |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.011 |
| Data reduction software | XDS (v.2015) |
| Data scaling software | XSCALE (November 3, 2014) |
| Phasing software | PHASER (v2.5.6) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.549 | 1.534 |
| High resolution limit [Å] | 1.496 | 1.496 |
| Rmerge | 0.056 | 0.412 |
| Number of reflections | 21841 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 83.1 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 8000, Hepes/sodium hydroxide |
