5KHD
Structure of 1.75 A BldD C-domain-c-di-GMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 36.160, 95.580, 99.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.977 - 1.750 |
R-factor | 0.1832 |
Rwork | 0.183 |
R-free | 0.18500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.036 |
RMSD bond angle | 3.138 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.977 |
High resolution limit [Å] | 1.750 |
Number of reflections | 17238 |
<I/σ(I)> | 10 |
Completeness [%] | 96.5 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Potassium/sodium phosphate, pH 6.5 |