5KGR
Spin-Labeled T4 Lysozyme Construct I9V1/V131V1 (30 days)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-19 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.031, 60.031, 95.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.169 - 1.473 |
| R-factor | 0.1673 |
| Rwork | 0.166 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c6t |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.805 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.170 | |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.049 | |
| Number of reflections | 34344 | |
| <I/σ(I)> | 8.86 | |
| Completeness [%] | 100.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2 M sodium/potassium phosphate, 150 mM sodium chloride, 100 mM 1,2-hexanediol, 3% 2-propanol |
