5KGO
Structure of K. pneumonia MrkH-c-di-GMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-01-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.573, 74.569, 135.538 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.285 - 2.900 |
R-factor | 0.2318 |
Rwork | 0.226 |
R-free | 0.28870 |
Structure solution method | MAD |
RMSD bond length | 0.028 |
RMSD bond angle | 1.721 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.285 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.800 | |
Number of reflections | 14247 | |
<I/σ(I)> | 5.2 | 1.2 |
Completeness [%] | 94.1 | 96.5 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 0.1 M Bis tris propane/HCl pH 6.5, 0.2 M sodium fluoride. |