5KFZ
Human DNA polymerase eta-DNA ternary complex: reaction first with 1 mM Mn2+ for 1800s then with 5 mM Mn2+ for 60s at 14 degree
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 98.460, 98.460, 82.250 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.733 - 1.440 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.480 | |
High resolution limit [Å] | 1.440 | 6.440 | 1.440 |
Rmerge | 0.050 | 0.028 | 0.715 |
Number of reflections | 81234 | ||
<I/σ(I)> | 23.06 | 75.39 | 2.83 |
Completeness [%] | 99.3 | 92.3 | 100 |
Redundancy | 7.5 | ||
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 298 | 17% PEG 2000MME, 0.1 M MES |