5KFX

Human DNA polymerase eta R61A-DNA ternary complex: reaction with 1 mM Mg2+ for 300s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.480, 98.480, 82.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.980 - 1.520
R-factor	0.183
Rwork	0.181
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.560
High resolution limit [Å]	1.520	1.520
Rmerge	0.055	0.682
Number of reflections	69539
<I/σ(I)>	25.57	2.5
Completeness [%]	99.9	99.9
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	17% PEG 2000MME, 0.1 M MES