5KDX
IMPa metallopeptidase in complex with T-antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-28 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.410, 133.100, 103.780 |
| Unit cell angles | 90.00, 103.12, 90.00 |
Refinement procedure
| Resolution | 48.120 - 2.400 |
| R-factor | 0.2038 |
| Rwork | 0.201 |
| R-free | 0.25415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kdv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.283 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.139 | 0.472 |
| Number of reflections | 102258 | |
| <I/σ(I)> | 7.1 | 2.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | PEG 3350, NaH2PO4, Bicine pH 9 |
