5KDW
IMPa metallopeptidase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-08 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.920, 132.730, 103.070 |
| Unit cell angles | 90.00, 103.81, 90.00 |
Refinement procedure
| Resolution | 100.090 - 1.850 |
| R-factor | 0.15481 |
| Rwork | 0.153 |
| R-free | 0.18638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kdv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.608 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.090 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.099 | 0.515 |
| Number of reflections | 219649 | |
| <I/σ(I)> | 9.5 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | PEG 3350, NaH2PO4, Bicine pH 9 |
