5KC7
Crystal structure of Cbln1 (Val55-Gly58 deletion mutant)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97625 |
Spacegroup name | I 21 3 |
Unit cell lengths | 187.450, 187.450, 187.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.098 - 7.035 |
R-factor | 0.2772 |
Rwork | 0.270 |
R-free | 0.35100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kc5 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.916 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.550 | 7.220 |
High resolution limit [Å] | 7.000 | 7.000 |
Rmerge | 0.048 | 0.394 |
Number of reflections | 1801 | |
<I/σ(I)> | 28.2 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.4 | 9.9 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.75 M 1,6-Hexanediol, 50 mM Tris pH 8.0 and 5 mM magnesium sulphate |