5KC6
Crystal structure of Cbln1 (Val55-Gly58 deletion mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97630 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.580, 170.450, 116.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.770 - 2.801 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kc5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.722 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1772) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.220 | 3.020 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.056 | 0.425 |
| Number of reflections | 14603 | |
| <I/σ(I)> | 21.7 | 5.6 |
| Completeness [%] | 73.4 | 19 |
| Redundancy | 6.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% (v/v) glycerol, 8.5% (v/v) isopropanol, 17% (w/v) polyethylene glycol 4000, 0.085 M Na.HEPES pH 7.5 and 0.02 M sodium bromide |
