5KBJ
Structure of Rep-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.200, 77.500, 100.700 |
| Unit cell angles | 80.80, 75.50, 71.90 |
Refinement procedure
| Resolution | 97.100 - 3.090 |
| R-factor | 0.2313 |
| Rwork | 0.225 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pql |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.641 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 97.100 |
| High resolution limit [Å] | 3.090 |
| Number of reflections | 28484 |
| <I/σ(I)> | 7 |
| Completeness [%] | 94.5 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 28% PEG 400, 0.1 M sodium/potassium phosphate (pH 6.9), 4% PEG 3350, and 10% polyethylene glycol |
