5KBA
Computational Design of Self-Assembling Cyclic Protein Homooligomers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.372, 48.817, 139.267 |
| Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
| Resolution | 19.907 - 2.601 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23880 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.738 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 19299 |
| <I/σ(I)> | 14.5 |
| Completeness [%] | 98.0 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.4 M Sodium Chloride, 0.1 M Tris-HCl pH 8.5 and 30 % PEG 3,350 |
