5K9O
Crystal structure of multidonor HV1-18+HD3-9 class broadly neutralizing Influenza A antibody 31.b.09 in complex with Hemagglutinin H1 A/California/04/2009
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 208.648, 208.648, 252.031 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.965 - 3.387 |
| R-factor | 0.294 |
| Rwork | 0.291 |
| R-free | 0.34560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wt5 3al4 3lzg 3ube 3ubj 3ubn 3ubq 3ztn 4jtv 4jtx 4juo 4lss |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.841 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.380 |
| Number of reflections | 29074 |
| <I/σ(I)> | 11.76 |
| Completeness [%] | 71.0 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M imidazole, pH 6.5, 0.1M MgCl2, 1.75M NaCl, and 15% PEG-3350 |
