5K9J
Crystal structure of multidonor HV6-1-class broadly neutralizing Influenza A antibody 56.a.09 isolated following H5 immunization.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.790, 103.758, 59.983 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.124 - 1.904 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bvk 4lss |
RMSD bond length | 0.004 |
RMSD bond angle | 0.770 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 25791 |
<I/σ(I)> | 18.29 |
Completeness [%] | 99.0 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M sodium acetate, pH 5.5, 25% (w/v) PEG-400 and 11% (w/v) PEG-8000 |