5K99

Crystal structure of microcin immunity protein MccF from Bacillus anthracis in complex with McC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 43
Unit cell lengths	118.275, 118.275, 55.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.266 - 1.500
R-factor	0.1331
Rwork	0.132
R-free	0.15330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jvo
RMSD bond length	0.011
RMSD bond angle	1.216
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	28.266
High resolution limit [Å]	1.500
Rmerge	0.032
Number of reflections	189029
<I/σ(I)>	19
Completeness [%]	96.0
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M Succinic acid ph 7.0, 15% Peg 3350