5K8M
Apo 5-nitroanthranilate aminohydrolase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 175.270, 175.270, 354.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.540 - 2.750 |
| R-factor | 0.1792 |
| Rwork | 0.178 |
| R-free | 0.21350 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.482 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 78.540 |
| High resolution limit [Å] | 2.540 |
| Number of reflections | 69712 |
| <I/σ(I)> | 10.41 |
| Completeness [%] | 94.8 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.07 M Monosodium phosphate, 0.83 M Dipotassium phosphate |
