5K8L
Crystal structure of ZIKV NS3 helicase in complex with GTP-gammar S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-04-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.860, 51.750, 66.200 |
| Unit cell angles | 90.00, 105.77, 90.00 |
Refinement procedure
| Resolution | 31.628 - 1.751 |
| R-factor | 0.1887 |
| Rwork | 0.186 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.865 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 42472 |
| <I/σ(I)> | 11.23 |
| Completeness [%] | 99.2 |
| Redundancy | 5.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG 6000, 5% MPD, 0.1M HEPES pH 7.5 |
