5K7J
Structure of designed zinc binding protein ZE2 bound to Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.07426 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.718, 79.029, 74.077 |
| Unit cell angles | 90.00, 95.70, 90.00 |
Refinement procedure
| Resolution | 28.620 - 1.390 |
| R-factor | 0.1977 |
| Rwork | 0.196 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a53 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.403 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.710 | 1.400 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.078 | 0.906 |
| Number of reflections | 91000 | |
| <I/σ(I)> | 28.1 | 2.1 |
| Completeness [%] | 99.7 | 95.8 |
| Redundancy | 8.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.10 M succinate 22% PEG 3350 |
